Solus Listeria monocytogenes ELISA

Manufacturer: PerkinElmer
HTTS ref.: HTS-SA-0068
HTDS ref.: SOLUS LISTERIA MONOCYTOGENES ELISA
Division: Sciences Analytiques

Gallery and technical drawings

Solus Listeria monocytogenes ELISA - Image 1

Solus Listeria monocytogenes ELISA - Image 2

Solus Listeria monocytogenes ELISA - Image 3

Solus Listeria monocytogenes ELISA - Image 4

Description

Avec la solution ELISA de Solus Listeria monocytogenes, vous obtenez un test très efficace qui donne des résultats en 51 heures, dont trois heures pour des résultats négatifs / présumés positifs après l’enrichissement. Les laboratoires ayant des exigences de débit d’échantillons plus élevées peuvent utiliser la méthode automatisée Solus pour améliorer le flux de travail du laboratoire, ce qui réduit considérablement le temps des techniciens et augmente la capacité de test.

Le kit de test Solus Listeria monocytogenes ELISA fournit aux laboratoires un outil facile à utiliser, rentable et très efficace pour la détection de la Listeria monocytogenes.

Détection de Listeria monocytogenes dans les échantillons alimentaires et environnementaux

Key features

Hautement fiable
Augmentation de l’efficacité du laboratoire
Réduction de la demande de ressources
Réduction des déchets
Haute efficacité lorsqu’il est automatisé
Temps du technicien considérablement réduit
Méthode de test de performance de l’Institut de recherche AOAC®

Technical specifications

P: (800) 762-4000 or 12 12 3 3
0,2ml: 6 6 6 6
Day 0: Day 1 Day 2 Day 3 Day 4
100 ml: 2 Read plates Read plates
30 °C: agents e.g. ALOA incubate for a
STEP 1: STEP 2 STEP 3 STEP 4 STEP 5 STEP 6 STEP 7
SUPP011: P.A.C. supplement 10 vials
Incubate: 3 3 Solus Automated ProcessSolus Automated Process
25 g food: Transfer 0.2 ml to 10 ml Take 1-2 ml heat
LISM-0096: Solus Listeria monocytogenes 96 wells
e.g. ALOA: incubate for a
in 225 ml: PAC supplemented kill and test
in 375 ml: 1 1
125 g food: 37 °C 24-28 hrs. +ve confirm
24-28 hrs.: Incubate -ve result or
Incubation: Incubation: Boil: 37 °C, 60 min. 37 °C, 60 min. 20-25 °C, 30 min. 450 nm
Swab/sponge: Incubate Incubate 35°C +ve confirm
10 ml/100 ml: Solus Automated ProcessSolus Automated Process2
MED039-0.5KG: Palcam broth 500 g
Product Code: Product Description Pack Size
SampleSample: 8 8 8 8
30 °C 24 hrs.: 4 4
100 ml25 g food: Add filter Streak onto 1 esculin Read plates Read plates
A B C D E F G H: A B C D E F G H A B C D E F G H A B C D E F G H
HIGH THROUGHPUT: EASY TO IMPLEMENT,
in 90 ml/225 ml: 30 °C 20 hrs. 35 °C 24 hrs.
per swab/sponge: 5
Negative Control: Positive Control Blank Well Sample wells Positive (After Substrate Addition) Positive (After Stop Solution Addition)
incubation after: 1 1
940 Winter Street: 1111 1111 5
Half Fraser broth: 5 5
PerkinElmer, Inc.: 1010 1010 1 1
+ve result confirm: Solus Automated ProcessSolus Automated Process
RESULTS IN 51 HOURS: WITH A SINGLE ASSAY
Workflow Comparison: Solus Listeria monocytogenes ELISA vs BAM Chapter 10
www.perkinelmer.com: 4 4 8 8
1 chromogenic plate,: Read plates or
FOR BETTER EFFICIENCY: EASY TO USE
Waltham, MA 02451 USA: 2 2 6 6
LISTERIA MONOCYTOGENES: TEST MULTIPLE MATRICES
Solus Automated Proces6: 6 35°C +ve confirm
BLEB + Pyruvate Incubate: selective 1 chromogenic plate, Read plates or
Solus Automated Process8: 8
the capacity for testing.: Efficient
1 2 3 4 5 36 7 8 9 1011 12: 132 3 4 5 6 7 8 9 1011 12 3 1 2 3 4 5 6 7 8 9 31011 12 1 2 3 4 5 6 7 8 9 1011 12
1 2 3 4 5 6 7 8 9 10 11 12: 1 2 3 4 5 106 7 8109 10 11 12 1 2 3 4 5 6 7 8
essential laboratory tasks.: detection ofListeria monocytogenes.
out on a Dynex DS2 instrument. A: LISM-0480 Solus Listeria monocytogenes 480 wells
the technician can walk away for: DS200 DS2 Automation 1
Half Fraser broth to supplemented: culture to into wells (plus controls) of conjugate 100 μl of substrate stop solution
up to three hours during each run,: Dynex DS2
single system processes two 96-well: FBH003/FBH005 Solus Half Fraser broth 2 kg,10 kg
Solus Automated Processfurther 24 hrs. @: -ve result or
and mix Palcam broth boiling tube Incubation: Incubation: Incubation: Read plate:
Presented in an immunoassay format, Solus Listeria: 51 hours, including three hours to negative/presumptive
Solus1Listeria monocytogenes2 3 4 5 6 7 8 9 10 11ELISA12: 1 2 3 4 5 6 7 8 9 10 11 12 12 12 1 2 3 4 5 6 7 8 9 10 11 12
monocytogenes ELISA is versatile and can be used manually or in: positive results post enrichment. Plus, when combined with
conjunction with automation. Performing to high standards, Solus: automation, you’ll benefit from an assay that’s:
Add sample to Transfer 0.2 ml culture Transfer1ml Pipette 100 μl sample: Add 100 μl Wash plate and add Add 100 μl of