Compteur automatisé de cellules Countess 3

Manufacturer: Thermo Fisher Scientific
HTTS ref.: HTS-SA-0356
HTDS ref.: COMPTEUR AUTOMATISE DE CELLULES IICOUNTESS
Division: Sciences Analytiques

Gallery and technical drawings

Compteur automatisé de cellules Countess 3 - Image 1

Compteur automatisé de cellules Countess 3 - Image 2

Description

HTDS vous propose deux compteurs de cellules automatisés hautes performances conçus pour répondre aux besoins de tout laboratoire en matière de comptage cellulaire précis et précis sans la corvée, la perte de temps, la subjectivité et la variabilité du comptage manuel des hémocytomètres. Développés par des biologistes cellulaires experts à l’aide de l’intelligence artificielle, les instruments Invitrogen Countess 3 et Countess 3 FL sont presque un paradoxe : des compteurs de cellules automatisés qui pensent comme un biologiste cellulaire. Les compteurs de cellules automatisés Countess 3 fournissent des comptages de cellules en fond clair et en fluorescence très précis et précis, même pour les types d’échantillons de cellules difficiles. Des fonctionnalités automatisées telles que la capture rapide, la sauvegarde automatique et les calculatrices intégrées ajoutent de la commodité et des gains de temps. Que vous comptiez les cellules manuellement ou que vous utilisiez un compteur automatisé de première génération, les compteurs de cellules Countess 3 représentent une amélioration significative de la précision du comptage et de l’efficacité du flux de travail.

Le compteur de cellules automatisé Countess 3 est une plate-forme d'analyse de paillasse équipée d'une optique de pointe, d'un autofocus complet et d'un logiciel d'analyse d'image pour une évaluation rapide des cellules en suspension.

Un compteur automatisé de cellules rapide, précis et facile d'utilisation

Key features

Précis: éliminez la subjectivité du comptage manuel des cellules et la variabilité d’un utilisateur à l’autre
Rapide: fournit un nombre total de cellules, signale la viabilité et mesure la taille moyenne des cellules en aussi peu que 10 secondes
Pratique – ne nécessite aucun nettoyage ni entretien de routine. Un écran tactile capacitif et une interface utilisateur simple permettent un démarrage rapide et nécessitent une formation minimale.

Technical specifications

A: Yes, the 1:1 dilution is automatically accounted for by the Q. Can I buy the Countess 3 FL instrument without
Q: Do the Countess 3 and Countess 3 FL Automated more concentrated samples take longer, but still less
Cy7: 710/40 809/81 Cy7, IRDye 800CW, DyLight 750 AMEP4667
10¹: 10²
A549: Human Lung
HeLa: Human Cervix
Name: Quantity Cat. No.
U2OS: Human Bone
U937: Human Blood
cube: (nm) (nm) dyes/fluorescent proteins Cat. No.
CAR T: Human Blood
Cells: 90.697%
HCASM: Human Smooth muscle
HepG2: Human Liver
Light: Excitation Emission Common compatible
MCF-7: Human Breast
Ramos: Human Blood
THP-1: Human Macrophage
the 1: 1 dilution of trypan blue to sample?
AsPC-1: Human Pancreas
CHO-K1: Hamster Ovary
HEK293: Human Kidney
Jurkat: Human Blood
SKBR-3: Human Breast
Cy5 2.0: 628/40 692/40 Cy5, Alexa Fluor 647, Alexa Fluor 660, DRAQ5 AMEP4956
GFP 2.0: 470/22 525/50 AMEP4951
RFP 2.0: 531/40 593/40 AMEP4952
YFP 2.0: 500/24 542/27 EYFP, AO (+DNA) AMEP4954
Autosave: * *
DAPI 2.0: 357/44 447/60 DAPI, Hoechst, BFP AMEP4950
FCS file: * *
50 slides: C10228
Cell line: Organism Cell type
NSCLC-146: Human Lung
500 slides: C10312
Countess 3: Countess 3 FL
Human PBMC: Human Blood
Mouse PBMC: Mouse Blood
microscope: Reusable Slide Counting Chamber Slides
be obtained.: Automated Cell Counters are static. They were developed
to pipetting.: autolighting, autocapture, and autosaving during this
10¹ Texas Red: 37.096%
Rat hepatocyte: Rat Liver
(A and B sides): (A and B sides) (A and B sides)
6. Adjust focus: 5. Use built-in calculator to calculate
Manual counting: Countess 3 Automated Cell Countess 3 Automated Cell
simple with a 1: 1 ratio of cell sample to stain.
Human hepatocyte: Human Liver
Human splenocyte: Human Spleen
Mouse hepatocyte: Mouse Liver
Single-cell data: * *
5. Adjust lighting: counter
HROC542TF fibroblast: Human Colorectal
Red Viability Stain.: Dead Cell Stain.
Texas Red 2.0 585/29: 628/32 AMEP4955
4. Mount on microscope: 4. Insert slide into Countess 3 3. Use built-in calculator to calculate
Automated Cell Counter: Automated Cell Counter
Countess Reusable Slide: 1 slide A25750
Countess reusable slide: *
Mouse spleen/T cell mix: Mouse Spleen and blood
Pre-dilution calculator: * *
with a hemocytometer and: Counter with Countess Counter with Countess Cell
• Counting 4 quadrants: • 2–3 minutes • ~1 minute
Brightfield rapid capture: * *
Fluorescence autolighting: N/A *
Saturated pixel indicator: N/A *
2. Clean and dry coverslip: 2. Clean coverslip 2. Insert slide into Countess 3
Machine-learning algorithm: * *
• Using autosave feature: • Using autosave feature
1 instrument plus 1 license: AMQAF2001
• Time to count 2 samples: • Time to count 2 samples • Time to count 2 samples
1,280 x 800 pixel LCD screen: * *
7-Aminoactinomycin D (7-AAD): Texas Red 2.0 or Cy5 2.0 A1310
ReadyCount Blue Nuclear Stain: 100 tests A49904
1. Clean and dry hemocytometer: 1. Clean reusable slide 1. Load sample into chamber slide
Fluorescence images and counts: N/A
ReadyCount Red Dead Cell Stain: 100 tests A49903
SYTOX Deep Red Dead Cell Stain: Cy5 2.0 S11381
SYTOX Green Nucleic Acid Stain: GFP 2.0 S7020
21 CFR Part 11 software support: * *
Countess 3 Automated Cell Counter: 1 instrument AMQAX2000
Wi-Fi–enabled cloud connectivity: * *
Countess 3 FL Automated Cell Counter: 1 instrument AMQAF2000
High-Power USB Wi-Fi Module (dongle): 1 module A26774
LIVE/DEAD Viability/Cytotoxicity Kit: L3224
Propidium Iodide ReadyProbes Reagent: RFP 2.0 or Texas Red 2.0 R37108
ReadyCount Green/Red Viability Stain: 100 tests A49905
Instrument plus 3 yr REX ext warranty: A49892
Instrument plus 3 yr ABRC ext warranty: A49893
Countess 3 Cell Counting Standard Slide: 1 slide A51876
Countess 3 Starter Pkg w/Rapid Exchange: 1 package plus 3 yr REX ext warranty A50298
• Built-in autolighting and autofocus: • Built-in autolighting and autofocus
Countess 3 21 CFR Part 11 Software Module: 1 license A51025
Countess 3 and 3 FL Reusable Slide Holder: 1 slide holder A48208
ReadyCount Green/Red Cell Viability Stain: GFP 2.0 and Texas Red 2.0 A49905
Countess 3 FL Starter Pkg w/ Rapid Exchange: 1 package plus 3 yr REX ext warranty A50299
Countess 3 and Countess 3 FL Automated Cell: cells as live, dead, and in clumps.
Countess 3 FL Automated Cell Counters count?: continue to learn with new cell samples?
CellEvent Caspase-3/7 Green Detection Reagent: GFP 2.0 C10423
Countess 3 Automated Cell Counter Starter Pkg: 1 package A49865
with the Countess 3 and Countess 3 FL Automated: counting with these small cells.
Countess 3 Automated Cell Counter w/ABRC Support: Instrument plus 3 yr ABRC ext warranty A49891
Countess 3 FL Automated Cell Counter Starter Pkg: 1 package A49866
ReadyProbes Cell Viability Imaging Kit, Blue/Red: R37610
iodide detected with Invitrogen EVOS GFP 2.0 and: Brightfield cell diameter (μm)
EVOS Texas Red 2.0 LED light cubes, respectively.: GFP cell brightness (0–255)
ReadyProbes Cell Viability Imaging Kit, Blue/Green: DAPI 2.0 and GFP 2.0 R37609
Countess 3 Automated Cell Counters utilize machine-: Upon insertion of the slide, the Countess instrument will
Cell Counters provide count results that account for: than 30 seconds total.
Counting samples from 1 x 10 to 1 x 10⁸ has been A: Yes, counting PBMCs can be performed in both
accuracy and precision is 1 x 10 to 1 x 10 cells/mL.: 3 and Countess 3 FL Automated Cell Counters?
Countess 3 and Countess 3 FL Automated Cell Counters.: light cubes?
and analysis algorithms. When considering differences: the result. The counting is only one element included
between samples, be careful to control other variables: in this timeframe, as the Countess 3 and Countess 3
learning algorithms to provide fast and accurate focus: automatically provide optimized illumination and focus
simply select the built-in dilution calculator option.: can easily be added to the Countess 3 FL instrument as
performed, but with greater count-to-count variability.: brightfield and fluorescence modes. As primary
CV values of <5% are routine due to the advanced focus A: Less than 30 seconds from sample insertion to saving
such as ensuring a homogeneous sample by mixing it prior: FL Automated Cell Counters also perform autofocus,
Jurkat cell viability using acridine orange and propidium: 5 10 20 29
and have been verified for use with several commonly used: Cells ranging in size from ~4 μm to 60 μm and cell
cell lines and primary cell types (Figure 1 and Table 2).: suspension density ranging from 1 x 10 to 1 x 10 cells/mL
If additional pre- or post-dilution calculations are needed,: 3 instrument—as a brightfield cell counter. Light cubes
determination as well as cell and viability counts. Countess: on the cells, which helps to minimize the user variation
fluorescence visualized in a scatter plot. Plots demonstrate: 100
The concentration noted on the results screen reflects that A: Yes, the Countess 3 FL instrument without light
of the original sample, not the trypan blue–diluted sample.: cubes installed will function exactly like the Countess
Figure 5. Jurkat cells treated with Invitrogen ReadyCount Green/: Figure 6. Jurkat cells treated with Invitrogen ReadyCount Red
brightfield count can be used to gate out noncellular debris GFP: 49.180%
3. Load sample into hemocytometer 3. Load sample into reusable slide: counter