Cytomètre en flux Attune CytPix

Manufacturer: Thermo Fisher Scientific
HTTS ref.: HTS-SA-0440
HTDS ref.: CYTOMETRE EN FLUX ATTUNE CYTPIX
Division: Sciences Analytiques

Description

Le cytomètre en flux Attune CytPix est un analyseur cellulaire avancé qui a des caractéristiques communes avec le modèle Attune NxT. Sa caractéristique distinctive est une caméra à fond clair à grande vitesse qui enregistre des images d’événements individuels lorsqu’ils traversent la cellule d’écoulement. La caméra et le logiciel de cytométrie Attune aident à garantir que les événements que vous analysez sont des cellules uniques, par opposition à des doublets, des amas ou des débris. Ceci est crucial dans les applications de recherche en thérapie cellulaire et génique, mais est utile dans presque toutes les expériences de cytométrie en flux pour aider les chercheurs à comprendre la morphologie de chaque population cellulaire identifiée pour l’analyse. Les images peuvent également aider à identifier les débris et à optimiser les protocoles.

Le cytomètre en flux Attune CytPix Flow Cytometer combine la focalisation acoustique avec une caméra à fond clair à grande vitesse pour une cytométrie en flux à haut débit simultanée et une imagerie à fond clair haute résolution

Cytomètre en flux amélioré par imagerie, pour une analyse simultanée des données de morphologie et de flux

Key features

Confirmez la précision en temps réel : Utilisez des images pour ajuster les portes et les paramètres de la caméra, et excluez les cellules atypiques et les débris pour un déclenchement plus robuste
Caractérisez davantage les populations cellulaires : Documenter des populations morphologiquement distinctes dans les protocoles existants tels que la détection de l’apoptose
Capturez les interactions cellule à cellule : Visualisez et différenciez les événements coïncidents
Visualisez les caractéristiques structurelles de grandes populations : Utilisez des preuves photographiques détaillées à haut débit
Découvrez les opportunités d’analyse : Basé sur la fluorescence, la dispersion et les caractéristiques morphologiques
Mesurez les paramètres d’image dérivés : Utilisation de la fonction d’analyse d’image du logiciel Attune Cytometric Software 6.0
Contrôle qualité à haut débit : Détectez rapidement les problèmes de qualité en ajoutant une imagerie rapide aux flux de travail de CQ de la culture cellulaire et surveillez les changements dans la morphologie cellulaire au fur et à mesure que la plaque est traitée

Technical specifications

GPU: NVIDIA™Quadro™P2200 NA
RAM: 64 GB 32 GB
Red: 637 100 140
Blue: 488 50 100
Laser: Wavelength (nm) Beam-shaping optics (BSO)* (mW) Diode power** (mW)
image: NA
Optics: Laser profile 10 x 50 μm flat-top laser provides robust alignment
Violet: 405 50 100
Weight: ~33 kg (73 lb) ~29 kg (64 lb)
Imaging: Image view image option allows viewing of individual images in the NA
Service: Field service or rapid exchange option
Voltage: User-adjustable
imaging: Detection limit Visually detect 800 nm particles NA
Computer: FCS format FCS 3.1, 3.0
Fluidics: Fluid level sensing Active
Green†: 532 100 140
Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates
Mounting: Mounted on side or placed behind
Overlays: Comparative analysis between samples; 3D view
Warranty: 1-year standard warranty; extended warranty options available
features: Correlates images to flow cytometry data by backgating all or
software: workspace for any cell population
Automated: processed, processable
Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
Flow rate: Precise flow rate control via software; no hardware adjustments
Processor: Intel™Core™i7 processor
Threshold: Up to 4 individual thresholds with user option to apply Boolean logic
detection: Forward scatter Photodiode detector with 488/10 nm bandpass filter
File types: FCS; JSON; TIF
Image size: 16-bit
Upgradable: Expand capability with laser upgrade purchase installed by field service
processing: Model types Leukocytes; beads
regulatory: Quality management Manufacturing standards comply with the requirements of ISO 13485:2003
500 μL/min: 1,000 μL/min
Laser power: Yellow 561 50 100
Performance: Theoretical resolution 0.6 μm NA
Pulse width: <50 ns NA
Simmer mode: Instant on/off reduces wear up to 10-fold; only on during data acquisition; hours of usage reported
wash cycles: 1,000 μL/min
Compensation: Full matrix in automated and manual modes; on-plot compensation tools for fine adjustment; use of tubes and wells
Installation: ranges 15–30°C (59–86°F)
Sample tubes: Accommodates 17 x 100 mm to 8.5 x 45 mm tubes
User account: Administrative creation of individual user accounts with designated roles; permissions for advanced settings;
Audible noise: <65 dBA at 1.0 m
Concentration: Direct concentration measurement without use of counting beads
Field of view: 29 x 29 μm2 to 74 x 74 μm2 NA
Fluid storage: external
Minimum depth: 39.5 cm (15.6 in.); allow 33 cm (13 in.) for the cytometer unit and 6.5 cm (2.6 in.)
Minimum width: 43 cm (17 in.); total width is 99 cm (39 in.) when attached to an Attune flow cytometer
Mixing method: Each well mixed via aspiration and dispensation of sample (no shaking)
Sample window: Window allows viewing of well progress; protective coating prevents exposure to ambient light during acquisition
Specification: Invitrogen™ CytKick™ Autosampler Invitrogen™ CytKick™ Max Autosampler
standard mode: standard mode
Live streaming: Live update of statistics during event acquisition up to 35,000 events/sec
Sample cooling: NA
administration: management of individual accounts; user time tracking and sample count
specifications: performance validation compliance with standardized procedure
Autocalibration: Regular 30-day intervals with system-initiated function
Sample delivery: Positive displacement syringe pump for volumetric analysis
Sample recovery: System able to return unused samples
Software layout: Fully customizable for each user account
Electronic pulse: Measured area; height and width pulse for all detectors
Heat dissipation: <150 W
Image backgating: NA
Laser excitation: 405 nm NA
Laser separation: 100 μm 150 μm
Operating system: Microsoft™Windows™10 software, 64-bit
Pixel resolution: 0.3 μm/pixel NA
Processing speed: Up to 1,000 images/second (varies with image complexity)
Size (W x D x H): ~43 x 33 x 41 cm (17 x 13 x 16 in.)
Extended fluidics: Available with Invitrogen™ Attune™ NxT External Fluid Supply (EFS); optional external fluid tank with 10 L fluid capacity
Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance required
Regulatory status: For Research Use Only
Window extensions: User-adjustable
Image capture rate: NA
Keyword management: User-defined or custom
Operating humidity: <80%, noncondensing
Particle/cell size: 5–20 μm
Total fluid volume: two 2 L tanks
Graphics resolution: ™ ™
Image dynamic range: 10-bit
Instrument tracking: Automated daily baseline and performance test with Levey-Jennings plots
Mixing optimization: Mixing optimized to preserve cell viability; number of mixing cycles optimized to match sample analysis volume
Robust installation: Units installed by engineer; preplanning checklist, delivery, and installation;
Acquisition settings: Documented in FCS files and maintained upon import
Image export options: NA
following conditions: use an Attune NxT Flow Cytometer with standard 0.5 mm blocking configuration, an Invitrogen™
Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
Minimum particle size: ™ ™ ™
Number of wash cycles: Up to 10 wash cycles (user-defined)
Software requirements: Invitrogen™Attune™Cytometric Software
Compatible plate types: • Customizable to accept other plate types
Fluorescence detectors: 14 individual detectors
Image capture settings: NA
Sample analysis volume: 20 μL to 4 mL
Electrical requirements: 100–240 VAC, 50/60 Hz, <300 W
Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
Objective magnification: 20x NA
dual-monitor capability: dual-monitor capability
Extended fluidics option: 10 L fluid configuration
Fluorescence sensitivity: ≤80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ≤30 MESF for PE, ≤70 MESF for APC
Instrument specification: Attune CytPix Flow Cytometer Attune NxT Flow Cytometer
Minimum clearance height: 74 cm (29.1 in.) above the mounting
2 x 8 TB SSD, 560 MB/sec;: 2 x 2 TB SATA 3.0 GB/sec, 8 MB data burst cache;
Area scaling factor (ASF): User-adjustable
Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
Carryover Single tube format: <1%
Plate and tube compatibility: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot
controller RAID1, integrated: controller RAID1, integrated
Minimum dead volume (single draw): 30 μL for 12.5–200 μL/min; 50 μL for 1,000 μL/min
Evaporation protection (foil cover): NA Yes
optional 405/10 + OD2 bandpass filter: optional 405/10 nm bandpass filter
PMT with default 488/10 nm bandpass fillter;: PMT with default 488/10 nm bandpass filter;
27 in. flat panel (1,920 x 1,080 resolution);: 23 in. flat panel (1,920 x 1,080 resolution);
1,000 μL samples with one mix and one rinse in: 1,000 μL samples with one mix and one rinse in
• 96 deep-well (flat, U-bottom, and V-bottom): • 96 deep-well (flat, U-bottom, and V-bottom)
• 384 deep-well (flat, U-bottom, and V-bottom): • 384 deep-well (flat, U-bottom, and V-bottom)
• <1.0% carryover for 12.5 μL and 25 μL samples: • <1.0% carryover for 12.5 μL and 25 μL samples
• <70 min per 96-well plate in standard mode with: one mix, and full analysis for each 20 μL sample at
• <42 min per 96-well plate in high-throughput mode: • ~22 min per 96-well plate in Boost mode; one rinse,
one rinse, and full analysis for each 20 μL sample at: one mix, and full analysis for each 20 μL sample at
• <0.5% carryover for 100 μL, 200 μL, 500 μL, and: • <0.5% carryover for 100 μL, 200 μL, 500 μL, and
• 96-well standard depth (flat, U-bottom, and V-bottom): • 96-well standard depth (flat, U-bottom, and V-bottom)
• 384-well standard depth (flat, U-bottom, and V-bottom): • 384-well standard depth (flat, U-bottom, and V-bottom)
~49 x 58 x 43 cm (19 x 23 x 17 in.), including fluid bottles: ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
Image measurement tool Elliptical tool to measure event areas in images in μm2: NA